Study on Lidocaine Inhibiting Proliferation, Migration and Invasion of Breast Cancer Cells by Modulating the MicroRNA-495-3p/Fibroblast Growth Factor 9 Axis

To investigate the mechanism of lidocaine in inhibiting proliferation, migration and invasion breast cancer cells. Human cell line Michigan Cancer Foundation-7 cells were treated with lidocaine. Cell proliferation was detected by 2,5-diphenyl-2H-tetrazolium bromide, Transwell, microRNA-495-3p expression quantitative reverse transcription polymerase chain reaction fibroblast growth factor 9, cyclin D1, matrix metalloproteinase 2 9 protein western blot. transfected inhibitor or overexpression vector. The effects silencing on human Transwell dual-luciferase reporter gene experiment confirmed relationship between targeting. Lidocaine could inhibit cells, promote microRNA- 495-3p Silencing decreased inhibitory effect MicroRNA-495-3p negatively regulated reversed inhibits up-regulating microRNA-495-3p, thus